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BINGO! Understanding Coronoavirus Isolation and Gene Sequencing


Scogan
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One puzzle I have been sorting out in my mind is the question; Has COVID been isolated and sequenced?  I had thought it was based on CDC saying so until I found these two papers. Now I am more in alignment with Mr. Icke.  

 

The first paper I found on the NIH website linked below:  Identification of Coronavirus Isolated from a Patient in Korea with COVID-19

 

This explains how Korea Isolated the virus:  Swab samples are cultured in Vero cells. "Isolation is confirmed through cytopathic effects, gene detection, and electron microscopy."  (English translation: Does it damage cells, does it replicate, are genes present (common CoV genes)  and under microscope does it look like a coronavirus)

 

Next, they sequenced the full virus genome:  "Using reverse transcriptase, cDNA was synthesized from RNA extracted from the cultured cell medium in which the virus was replicated. A next generation sequencing (NGS) library was constructed after amplifying the full-length genes of the isolates using the synthesized cDNA and primers designed based on published SARS-CoV-2 DNA sequence."  (In plain english:  Swab cultures detect what looks like a coronavirus then they confirm the presence of CoV genetic material (CoV is a large group of viruses that have been around a very long time. It is important to note that this does NOT isolate SARS CoV-2!  It isolates common CoV)  They then extract replicated cell cultures and fill in the missing genome gaps with DESIGNED PRIMERS from the DNA genome handbook on SARS Cov-2 (Initially provided by China). DESIGNED PRIMERS ARE SYNTHETIC.  A designed Primer is made in a lab to create short gene segments.  You can read more about designed primers here:  https://www.addgene.org/protocols/primer-design/#:~:text=One needs to design primers that are complementary,when adding a nucleotide one at a time. 

 

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7045880/

 

 

The second paper I found on Reasearchgate: WUHAN COVID-19 SYNTHETIC ORIGINS AND EVOLUTION

 

This confirms that the SARS Cov-2 virus is partially a synthetic genome:  "Abstract   The main result of this updated release is the formal proof that 2019-nCoV coronavirus is partially a SYNTHETIC genome."

 

https://anatomiadaconsciencia.files.wordpress.com/2020/04/wuhan-covid-19-synthetic-origins-and-evolution.pdf

 

 

My conclusion:  This is not a virus from nature.  Since when does a virus from nature require DESIGNED SYNTHETIC PRIMERS to fill in the gaps?  SARS CoV-2 is a lab made virus.  People are not being sickened with SARS CoV-2 if the only way they can sequence the complete genome is in a lab using designed primers.  This is insane.  

 

Please tell me if I got it wrong here.  I am not a virologist.  

 

 

 

 

 

 

 

 

Edited by Scogan
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More confirmation that SARS CoV-2 genomes are synthetic constructions.

 

"The generation of the SARS-CoV-2 from chemically synthesized DNA could bypass the limited availability of virus isolates and would furthermore enable genetic modifications and functional characterization."

 

 

https://www.nature.com/articles/s41586-020-2294-9

 

 

There is plenty of online papers where China did exactly that.  

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