BINGO! Understanding Coronoavirus Isolation and Gene Sequencing

[Scogan]

One puzzle I have been sorting out in my mind is the question; Has COVID been isolated and sequenced?  I had thought it was based on CDC saying so until I found these two papers. Now I am more in alignment with Mr. Icke.  

 

The first paper I found on the NIH website linked below:  Identification of Coronavirus Isolated from a Patient in Korea with COVID-19

 

This explains how Korea Isolated the virus:  Swab samples are cultured in Vero cells. "Isolation is confirmed through cytopathic effects, gene detection, and electron microscopy."  (English translation: Does it damage cells, does it replicate, are genes present (common CoV genes)  and under microscope does it look like a coronavirus)

 

Next, they sequenced the full virus genome:  "Using reverse transcriptase, cDNA was synthesized from RNA extracted from the cultured cell medium in which the virus was replicated. A next generation sequencing (NGS) library was constructed after amplifying the full-length genes of the isolates using the synthesized cDNA and primers designed based on published SARS-CoV-2 DNA sequence."  (In plain english:  Swab cultures detect what looks like a coronavirus then they confirm the presence of CoV genetic material (CoV is a large group of viruses that have been around a very long time. It is important to note that this does NOT isolate SARS CoV-2!  It isolates common CoV)  They then extract replicated cell cultures and fill in the missing genome gaps with DESIGNED PRIMERS from the DNA genome handbook on SARS Cov-2 (Initially provided by China). DESIGNED PRIMERS ARE SYNTHETIC.  A designed Primer is made in a lab to create short gene segments.  You can read more about designed primers here:  https://www.addgene.org/protocols/primer-design/#:~:text=One needs to design primers that are complementary,when adding a nucleotide one at a time. 

 

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7045880/

 

 

The second paper I found on Reasearchgate: WUHAN COVID-19 SYNTHETIC ORIGINS AND EVOLUTION

 

This confirms that the SARS Cov-2 virus is partially a synthetic genome:  "Abstract   The main result of this updated release is the formal proof that 2019-nCoV coronavirus is partially a SYNTHETIC genome."

 

https://anatomiadaconsciencia.files.wordpress.com/2020/04/wuhan-covid-19-synthetic-origins-and-evolution.pdf

 

 

My conclusion:  This is not a virus from nature.  Since when does a virus from nature require DESIGNED SYNTHETIC PRIMERS to fill in the gaps?  SARS CoV-2 is a lab made virus.  People are not being sickened with SARS CoV-2 if the only way they can sequence the complete genome is in a lab using designed primers.  This is insane.  

 

Please tell me if I got it wrong here.  I am not a virologist.  

 

 

 

 

 

 

 

 

Edited May 9, 2021 by Scogan
spelling error

[Scogan]

More confirmation that SARS CoV-2 genomes are synthetic constructions.

 

"The generation of the SARS-CoV-2 from chemically synthesized DNA could bypass the limited availability of virus isolates and would furthermore enable genetic modifications and functional characterization."

 

 

https://www.nature.com/articles/s41586-020-2294-9

 

 

There is plenty of online papers where China did exactly that.  

[Scogan]

Adding this report stating Electron Microscopy (used in Isolating viruses) has many problems with misidentification.  It should be noted there is no way to visually confirm SARS CoV2 under EM.  All that is distinguishable is a Coronavirus (Halo feature).

 

 

 

https://wwwnc.cdc.gov/eid/article/27/4/20-4337_article

[Grumpy Owl]

I think most of this has already been covered in the following topic: