Jump to content

BINGO! Understanding Coronoavirus Isolation and Gene Sequencing


Recommended Posts

One puzzle I have been sorting out in my mind is the question; Has COVID been isolated and sequenced?  I had thought it was based on CDC saying so until I found these two papers. Now I am more in alignment with Mr. Icke.  


The first paper I found on the NIH website linked below:  Identification of Coronavirus Isolated from a Patient in Korea with COVID-19


This explains how Korea Isolated the virus:  Swab samples are cultured in Vero cells. "Isolation is confirmed through cytopathic effects, gene detection, and electron microscopy."  (English translation: Does it damage cells, does it replicate, are genes present (common CoV genes)  and under microscope does it look like a coronavirus)


Next, they sequenced the full virus genome:  "Using reverse transcriptase, cDNA was synthesized from RNA extracted from the cultured cell medium in which the virus was replicated. A next generation sequencing (NGS) library was constructed after amplifying the full-length genes of the isolates using the synthesized cDNA and primers designed based on published SARS-CoV-2 DNA sequence."  (In plain english:  Swab cultures detect what looks like a coronavirus then they confirm the presence of CoV genetic material (CoV is a large group of viruses that have been around a very long time. It is important to note that this does NOT isolate SARS CoV-2!  It isolates common CoV)  They then extract replicated cell cultures and fill in the missing genome gaps with DESIGNED PRIMERS from the DNA genome handbook on SARS Cov-2 (Initially provided by China). DESIGNED PRIMERS ARE SYNTHETIC.  A designed Primer is made in a lab to create short gene segments.  You can read more about designed primers here:  https://www.addgene.org/protocols/primer-design/#:~:text=One needs to design primers that are complementary,when adding a nucleotide one at a time. 





The second paper I found on Reasearchgate: WUHAN COVID-19 SYNTHETIC ORIGINS AND EVOLUTION


This confirms that the SARS Cov-2 virus is partially a synthetic genome:  "Abstract   The main result of this updated release is the formal proof that 2019-nCoV coronavirus is partially a SYNTHETIC genome."





My conclusion:  This is not a virus from nature.  Since when does a virus from nature require DESIGNED SYNTHETIC PRIMERS to fill in the gaps?  SARS CoV-2 is a lab made virus.  People are not being sickened with SARS CoV-2 if the only way they can sequence the complete genome is in a lab using designed primers.  This is insane.  


Please tell me if I got it wrong here.  I am not a virologist.  









Edited by Scogan
spelling error
Link to comment
Share on other sites

More confirmation that SARS CoV-2 genomes are synthetic constructions.


"The generation of the SARS-CoV-2 from chemically synthesized DNA could bypass the limited availability of virus isolates and would furthermore enable genetic modifications and functional characterization."






There is plenty of online papers where China did exactly that.  

Link to comment
Share on other sites

Join the conversation

You can post now and register later. If you have an account, sign in now to post with your account.
Note: Your post will require moderator approval before it will be visible.

Reply to this topic...

×   Pasted as rich text.   Paste as plain text instead

  Only 75 emoji are allowed.

×   Your link has been automatically embedded.   Display as a link instead

×   Your previous content has been restored.   Clear editor

×   You cannot paste images directly. Upload or insert images from URL.

  • Create New...