KARY MULLIS, the inventor of the PCR test

[Beaujangles]

Whatthefoxhat said:  Mainly because the vast majority of posters here (myself included) do not have an intimate understanding of the medical and biological terms used ,the threads are,and don't be personally offended by this,boring,and it ends up being a pissing contest on who can use the most incomprehensible words and citation after citation of documents that require an intimate understanding of the terms used

 

We KNOW the pcr test is shit,we KNOW it has been used abused and flogged to death and whilst you continue to debate till the cows come home there are people dying,not of the pcr test but of vaccine related injuries.

 

How about going out tomorrow to an event and start the debate there? perhaps educate a few people that are not aware ?

 

The pcr test may appear to be the cornerstone of bringing the whole house of cards crashing down but i think that bus went past the stop long ago and they discontinued the service

 

 

I am not offended...my medical past employment stands me in good stead. I educate people regularly thank you. The PCR test is the cornerstone of this scam... and as for the bus...the key is to keep jumping on the next bus and making sure it arrives at the next stop. I notice that you often like to minimise such important issues... but being negative and defeatist doesnt help really. Focus of this thread is Kary Mullis and PCR... not whether or not you or others have missed the bus...please dont take offence.

[zarkov]

 

https://uncoverdc.com/2020/04/07/was-the-covid-19-test-meant-to-detect-a-virus/

 

excerpt:

 

What do we mean when we say a person “tests positive” for Covid-19?

We don’t actually mean they have been found to “have” it.

We’ve been hijacked by our technologies, but left illiterate about what they actually mean. In this case, I am in the rare position of having known, spent time with, and interviewed the inventor of the method used in the presently available Covid-19 tests, which is called PCR, (Polymerase Chain Reaction.)

His name was Kary B. Mullis, and he was one of the warmest, funniest, most eclectic-minded people I ever met, in addition to being a staunch critic of HIV “science,” and an unlikely Nobel Laureate, i.e. a “genius.”

R

One time, in 1994, when I called to talk to him about how PCR was being weaponized to “prove,” almost a decade after it was asserted, that HIV caused AIDS, he actually came to tears.

The people who have taken all your freedoms away in recent weeks, they’re social engineers, politicians, globalist thought leaders, bankers, WHO fanatics, and the like. Their army is composed of “mainstream media,” which is now literally a round-the-clock perfect propaganda machine for the Gates-led Pandemic Reich.

Kary Mullis was a scientist. He never spoke like a globalist, and said once, memorably, when accused of making statements about HIV that could endanger lives: “I’m a scientist. I’m not a lifeguard.” That’s a very important line in the sand.  Somebody who goes around claiming they are “saving lives,” is a very dangerous animal, and you should run in the opposite direction when you encounter them. Their weapon is fear, and their favorite word is “could.” They entrap you with a form of bio-debt, creating simulations of every imaginable thing that “could” happen, yet hasn’t. Bill Gates has been waiting a long time for a virus with this much, as he put it, “pandemic potential.” But Gates has a problem, and it’s called PCR.

Of Mullis’ invention, Polymerase Chain Reaction, the London Observer wrote:

“Not since James Watt walked across Glasgow Green in 1765 and realized that the secondary steam condenser would transform steam power, an inspiration that set loose the industrial revolution, has a single, momentous idea been so well recorded in time and place.”

What does HIV have to do with Covid-19?

PCR played a central role in the HIV war (a war you don’t know about, that lasted 22 years, between Globalist post-modern HIV scientists and classical scientists.) The latter lost the war. Unless you count being correct as winning. The relentless violence finally silenced the opposition, and it seemed nobody would ever learn who these scientists were, or why they fought this thing so adamantly and passionately.

[zarkov]

 

 

https://fort-russ.com/2020/06/covid19-pcr-tests-are-scientifically-meaningless/

 

By Torsten Engelbrecht and Konstantin Demeter for Off-Guardian 

Lockdowns and hygienic measures around the world are based on numbers of cases and mortality rates created by the so-called SARS-CoV-2 RT-PCR tests used to identify “positive” patients, whereby “positive” is usually equated with “infected.”

But looking closely at the facts, the conclusion is that these PCR tests are meaningless as a diagnostic tool to determine an alleged infection by a supposedly new virus called SARS-CoV-2.

 

UNFOUNDED “TEST, TEST, TEST,…” MANTRA

At the media briefing on COVID-19 on March 16, 2020, the WHO Director General Dr Tedros Adhanom Ghebreyesus said:

We have a simple message for all countries: test, test, test.”

The message was spread through headlines around the world, for instance by Reuters and the BBC.

Still on the 3 of May, the moderator of the heute journal — one of the most important news magazines on German television— was passing the mantra of the corona dogma on to his audience with the admonishing words:

Test, test, test—that is the credo at the moment, and it is the only way to really understand how much the coronavirus is spreading.”

This indicates that the belief in the validity of the PCR tests is so strong that it equals a religion that tolerates virtually no contradiction.

[zarkov]

https://theinfectiousmyth.com/coronavirus/RT-PCR_Test_Issues.php

Issues with the RT-PCR Coronavirus Test

David Crowe
April 23, 2020
Version 3

This is an analysis of the so-called coronavirus test, based on RT-PCR technology. It is based significantly on my recent reading of a 2017 article on potential problems with RT-PCR by Professor Stephen Bustin, a world expert, a podcast that I recently conducted with him and the MIQE guidelines for operating and reporting on RT-PCR data. This article does not question whether the RNA used in the test is viral or endogenous. If the RNA is not viral, then clearly the RT-PCR coronavirus test is of no value. This web page does not contain references, for those you should consult the fully referenced Coronavirus Panic Critique.

The PCR Cycle Number

The PCR algorithm is cyclical. At each cycle it generates approximately double the amount of DNA (which, in RT-PCR, corresponding to the RNA that the process started with). When used as a test you don’t know the amount of starting material, but the amount of DNA at the end of each cycle will be shown indirectly by fluorescent molecules that are attached to the probes. The amount of light produced after every step will then approximately double, and when it reaches a certain intensity the process is halted and the sample is declared positive (implying infected). If, after a certain number of cycles, there is still not sufficient DNA, then the sample is declared negative (implying not infected). This cycle number (Ct) used to separate positive from negative is arbitrary, and is not the same for every organization doing testing. For example, there is a paper published that reported using 36 as the cutoff for positive, 37-39 as indeterminate, requiring more testing, and above 39 as negative. Another paper used 37 as the cutoff, with no intermediate zone. In a list of test kits approved by the US FDA one manufacturer each recommended 30 cycles, 31, 35, 36, 37, 38 and 39. 40 cycles was most popular, chosen by 12 manufacturers, and one each recommended 43 and 45.

Meaning of the Ct

Implicit in using a Ct number is the assumption that approximately the same amount of original RNA (within a multiple of two) will produce the same Ct number. However, there are many possibilities for error in RT-PCR. There are inefficiencies in extracting the RNA, even larger inefficiencies in converting the RNA to complementary DNA (Bustin noted that efficiency is rarely over 50% and can easily vary by a factor of 10), and inefficiencies in the PCR process itself. Bustin, in the podcast, described reliance on an arbitrary Ct number as “absolute nonsense, it makes no sense whatsoever”. It certainly cannot be assumed that the same Ct number on tests done at different laboratories indicates the same original quantity of RNA.

Limits on Cycles

Professor Bustin stated that cycling more than 35 times was unwise, but it appears that nobody is limiting cycles to 35 or less (the MIQE guidelines recommend less than 40). Cycling too much could result in false positives as background fluorescence builds up in the PCR reaction.

Ct and Number of Positive Tests

The Ct cycle number will significantly influence the number of positive tests. If the Ct was changed from 37 to 35 there would be fewer positive tests, and if changed to 39 there would more positive tests. Even if the Ct number was standardized, it would still have different meaning depending on the specific machines, chemicals and procedures used by different labs, and even within the same lab changes could still be found between different runs of samples. Without simultaneously amplifying a known quantity of ‘spiked’ RNA, it cannot be assumed that with consistent Ct numbers can be used to consistently provide a boundary between positive and negative.

Is the Amount Meaningful?

If the process is efficient, a large number of cycles could detect as little as three molecules of RNA. If there are people who had such a small amount of virus in their body, causing no health problems, they would still test positive.

Is the Virus Functional?

If there are only parts of viruses present, or defective virus particles, that are not infectious, they would still produce positive tests. The tests do not prove that pathogenic, replicable virus is present.

Can RT-PCR Distinguish Infected from Uninfected

No.

How RT-PCR Works in More Detail

The following steps are used to test for particular RNA:

1. RNA must be extracted from a sample. This must be done carefully to ensure that DNA is eliminated, and that chemicals that might inhibit further steps are not included. It is impossible to ensure absolute purity of the RNA.
2. RNA must be converted to complementary DNA (cDNA). This uses the enzyme Reverse Transcriptase and is never terribly efficient (50%). The amount of DNA produced can vary significantly, depending on numerous factors, perhaps by a factor of 10 (it used to be a factor of 100).
3. In the PCR part of the process, cDNA is present with primers and a probe (and possibly some stray DNA from the sample). The primers delimit the beginning and the ending of the cDNA that is intended to be duplicated. The probe helps ensure that RNA is only duplicated if it matches the primers (which are quite short) and the probe. At each cycle of this process (PCR proper) the amount of DNA is approximately doubled. Fluorescent markers are attached to the probe so that, at each step, the amount of light can be used to estimate how much DNA has been generated.
4. Optionally, the resulting DNA can be sequenced to determine exactly what the bases (‘string of four different DNA beads’) are.

Errors and inefficiencies can occur at every step. It is not possible to actually estimate quantities unless the reaction is ‘spiked’ with a known amount of a different RNA, which is also duplicated. Then the PCR cycle number can be roughly correlated with the original quantity of material.

Is There Proof There Are Problems, Or Is This Just a Hypothesis?

There are now several papers that illustrate essentially impossible testing results. A paper from China reported on consecutive testing results, defined as either Negative (N), Positive (P) or Dubious (D, presumably intermediate). Results for 29 people with inexplicable results out of about 600 patients were: 1 DDPDD 2 NNPN 3 NNNPN 4 DNPN 5 NNDP 6 NDP 7 DNP 8 NDDPN 9 NNNDPN 10 NNPD 11 DNP 12 NNNP 13 PPNDPN 14 PNPPP 15 DPNPNN 16 PNNP 17 NPNPN 18 PNP 19 NPNP 20 PNPN 21 PNP 22 PNP 23 PNP 24 PNDDP 25 PNPNN 26 PNPP 27 PNP 28 PNPN 29 PNP. A study from Singapore did tests almost daily on 18 patients and the majority went from Positive to Negative back to Positive at least once, and up to four times in one patient. In China they have found that 5-14% of patients who have been cleared, with two consecutive negative tests, have later tested positive again, usually without new symptoms. In South Korea they recently reported 91 such patients. A 68 year old Chinese man went to hospital with symptoms, and tested positive. After his symptoms resolved and he tested negative twice he was released. But he tested positive again, and was readmitted, was released again, tested positive again, was readmitted, and then was released for a third time.

Conclusions

RT-PCR testing for the Coronavirus seems to be designed to produce as many positive tests as possible. The fear of missing a true positive is so great that those designing the specific testing methodology based on RT-PCR completely ignore the risk of false positives. False positives make the epidemic appear larger, and justify the complete shutdown of the economy, locking people in their own homes, and destroying just about everything in the lives of people that brings them joy, such as playing ball in the park, going for coffee with a friend, going to the theater or a sports event, going swimming, going to the county fair.

 

[wideawake]

17 hours ago, Beaujangles said:

Kary Mullis did not say C19 was a real virus.

 

 

 

My bad and thank you for pointing that out. Kary talks about viruses in general and HIV, not convid.

The point I was trying to get across was that they (Judy and Kary) were saying the same thing about Faulsi and viruses but I know Kary wasn't talking about C19 and Judy included C19.

I appreciate that you brought it up as I now notice that I included C19 in Kary's statement which isn't true.

There's enough false information out there about this scamdemic, there's no need to copycat CNN. 

 

 

[Beaujangles]

Another video of Kary Mullis...relating to scientists, climate change and so on

 

 

https://www.bitchute.com/video/7bJv3BHUrrKJ/

[TheConsultant]

9 hours ago, Beaujangles said:

Another video of Kary Mullis...relating to scientists, climate change and so on

 

 

https://www.bitchute.com/video/7bJv3BHUrrKJ/

an actual scientist who also looks like he would be fun to have a beer with. 

[Beaujangles]

3 hours ago, TheConsultant said:

an actual scientist who also looks like he would be fun to have a beer with. 

 

Yess... seems like he was a very nice, honest guy who knew what was really going on. I'd have a beer with him too...and I dont drink beer...LOL